A simple, accurate and economical UV-Spectrophotometric method has been developed for routine analysis of Moxifloxacin Hydrochloride (MOX) in ophthalmic solution. In saline phosphate buffer (PBS) pH 7.4 and simulated tear fluid (STF) pH 7.4, MOX showed maximum absorbance at 287.0 nm. In this method MOX obeyed linearity in the concentration range of 2 - 20 μg/mL (r2 > 0.99). Proposed methods were applied for ophthalmic solution and amounts of MOX estimated by this method were 99.93 ± 1, 99.36 ± 1 in phosphate buffer and in simulated tear fluid respectively. Methods were validated statistically.
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